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Image Search Results
Journal: PLoS Genetics
Article Title: Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells
doi: 10.1371/journal.pgen.1005932
Figure Lengend Snippet: a . Schematic comparing the reprogramming of polyclonal and monoclonal cells. Polyclonal cells such as fibroblasts (left panel) give rise to iPSCs which do not share a majority of mutations since they are derived from different progenitors. In contrast, iPSCs derived from monoclonal cells (right panel) such as EPCs share a proportion of their mutations and carry private mutations specific to each line. b, c . Exome sequencing of iPSCs generated using fibroblasts from two different individuals, a 65-year-old alpha-1 antitrypsin deficiency patient (AATD) ( b ) and a healthy subject, S2 ( c ). The data for iPSC-B were taken from our previous work . Each column represents one SNV in the indicated gene. Duplicated genes indicate two adjacent SNVs. Green, mutation absent; pink, mutation present. See and Tables for the complete description. d, e . Exome sequencing of iPSC lines generated using monoclonal EPCs from the same AATD patient in b as well as a healthy subject, S7 ( e ). Orange, mutation detected by amplicon resequencing. See and Tables for the complete description.
Article Snippet: Briefly, the cells were cultured on irradiated mouse embryonic fibroblast (MEF) feeder layers in
Techniques: Derivative Assay, Sequencing, Generated, Mutagenesis, Amplification